Lab Facility
Laboratory and Bio-repository Facilities at Centre for Chronic Disease Control in collaboration with Public Health Foundation of India
Need for an in-house laboratory
- Quality assurance
- Cost effectiveness
- Systematic bio-banking for future research activities
Infrastructure
Laboratory is equipped with high-ethroughput equipments to carry out biochemical analyse
| Facilities | Company |
|---|---|
| Electrochemiluminescence autoanalyzer (E411) | Roche |
| HbA1c analyzer | Bio-Rad |
| Electrolyte analyzer | Caretium |
| ELISA reader | Bio-Rad |
| Refrigerated centrifuge | REMI |
Table centrifuges, water baths, incubators, single channel and multi channel pipettes, magnetic stirrer, vortex machines, pH meter, weighing balance, water purification system etc.
Work is carried out by science graduates/ post graduates with training in Medical Laboratory Technology and Biotechnology with many years of experience.
Parameters
Clinical Chemistry
- Glucose
- Lipid Profile
- Kidney function tests
- Liver function tests
- Urine Chemistry
Other markers of Cardio-metabolic disease
- Insulin
- HbA1c
- Apo A1
- Apo B
Specialized tests
- Cytokines
- Cotinine
- Markers of Chronic Kidney Disease
Quality Assurance
Internal
- Two levels of internal quality control samples run with every batch of samples
- Westgard rules are followed for the rejection of runs
External
- RIQAS program for Clinical Chemistry, Extended Lipids (ApoA1, Apo B) & HbA1c
- UKNEQAS program for Insulin
Work from Jan 2011 till date …….
Studies Completed
- Andhra Pradesh Parents & Children Study (APCAPS)
- Cardio-metabolic Risk Reduction Surveillance Study (CARRS) – cohort 1 (Baseline, 2nd F-up), cohort-2
- Dual Energy X-ray Absorptiometry (DEXA) study
- ICMR Urban study
- Solan Surveillance Study (SSS)
- UDAY – Sonepat & Vizag
- Salt study
- Cytokine analysis
Bio-repository
- Systematic storage of samples
- Range of stored samples: Serum, Fluoride plasma, EDTA plasma, Whole blood, Buffy coat, RBCs, Spot Urine, Blood spots on filter paper
- Biological samples from 14 different studies: IMS, DXA,CARRS, ICMR-Urban Study, UDAY, Solan Surveillance Study, Salt Study, etc.
Facilities
- Ultra-low Deep freezers (-80° C): 28
- Deep freezers (-20° C): 4
- Refrigerators: 5
Maintenance
- 24-hr power back-up
- Temperature monitoring
Future Directions
- Expansion of laboratory in terms of infrastructure and analysis carried out to better facilitate research at PHFI
- Expand and better facilitate the bio-repository to meet the ever- increasing needs of ongoing and future studies
Our Practices
- Standard Operating Procedures for laboratory activities
- Log of all activities maintained
- Good Laboratory Practices followed
- Electronic data transfer for majority of analysis
- Periodic Evaluation and Re-training of lab staff
- Regular calibration and maintenance of equipments